Review



pacc 222  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Promega pacc 222
    (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.
    Pacc 222, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacc 222/product/Promega
    Average 90 stars, based on 1 article reviews
    pacc 222 - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "PacC-dependent adaptation and modulation of host cellular pH controls hemibiotrophic invasive growth and disease development by the rice blast fungus"

    Article Title: PacC-dependent adaptation and modulation of host cellular pH controls hemibiotrophic invasive growth and disease development by the rice blast fungus

    Journal: bioRxiv

    doi: 10.1101/2020.06.22.164590

    (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.
    Figure Legend Snippet: (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.

    Techniques Used: Expressing, Cell Culture, Purification, Binding Assay, Incubation, Clear Native PAGE, Mutagenesis, Infection

    (A) Infected plant cells are alkalinized during the early biotrophic growth of M. oryzae and then become acidified during the later necrotrophic growth. (B) During biotrophic growth, the PacC 559 and PacC 222 transcription factor isoforms localize to the nucleus, where PacC 559 acts as a transcriptional repressor to repress expression of genes associated with conidiation and necrotrophic growth, including PRG1 , HTF1 , and PIG1 while PacC 222 acts as a transcriptional activator to activate genes associated with biotrophic growth. (C) As host cells become acidified and lose viability, the PacC functional isoforms exit from the nucleus thereby de-repressing expression of genes related to necrotrophic growth and conidiation.
    Figure Legend Snippet: (A) Infected plant cells are alkalinized during the early biotrophic growth of M. oryzae and then become acidified during the later necrotrophic growth. (B) During biotrophic growth, the PacC 559 and PacC 222 transcription factor isoforms localize to the nucleus, where PacC 559 acts as a transcriptional repressor to repress expression of genes associated with conidiation and necrotrophic growth, including PRG1 , HTF1 , and PIG1 while PacC 222 acts as a transcriptional activator to activate genes associated with biotrophic growth. (C) As host cells become acidified and lose viability, the PacC functional isoforms exit from the nucleus thereby de-repressing expression of genes related to necrotrophic growth and conidiation.

    Techniques Used: Infection, Expressing, Functional Assay



    Similar Products

    primary antibodies against pacc ser212 222  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc primary antibodies against pacc ser212 222
    Primary Antibodies Against Pacc Ser212 222, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against pacc ser212 222/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    primary antibodies against pacc ser212 222 - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier
    pacc 222  (Promega)
    90
    Promega pacc 222
    (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.
    Pacc 222, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacc 222/product/Promega
    Average 90 stars, based on 1 article reviews
    pacc 222 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.

    Journal: bioRxiv

    Article Title: PacC-dependent adaptation and modulation of host cellular pH controls hemibiotrophic invasive growth and disease development by the rice blast fungus

    doi: 10.1101/2020.06.22.164590

    Figure Lengend Snippet: (A) Expression of PAG1 in mycelium of WT P131, Δ pacC , NGP239, and NGP559. For the Q-PCR, the expression level of P131 cultured at pH 5.5 was arbitrarily set to 1. (B) PacC binds to GCCAAG motifs in the PAG1 promoter. Purified GST-PacC 222 protein was used to detect binding of putative PacC binding motifs. Probes 1 and 2 contain predicted PacC-binding motifs and were prepared by labelling with 32 P-dCTP and incubated with GST-PacC 222 for 30 min, before loading a native-PAGE gel. For competition experiments, 100x or 10x concentrations of un-labelled Probe 1 were mixed with the GST-PacC 222 protein for 30 min before incubation with 32 P-dCTP-labelled probes. (C) WT and Δ pag1 are same in colony growth on OTA plates. (D) WT and Δ pag1 are equivalent in conidiation. (E) Reduced virulence of Δ pag1 mutant compared to WT. (F) Infection assays of WT and Δ pag1 on barley epidermis. (G) Arrested biotrophic growth of Δ pag1 compared to WT. Bar = 25 μm.

    Article Snippet: GST-fused PacC 559 , PacC 222 and PacC 80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E. coli BL21DE3 and purified, as described in supplementary data.

    Techniques: Expressing, Cell Culture, Purification, Binding Assay, Incubation, Clear Native PAGE, Mutagenesis, Infection

    (A) Infected plant cells are alkalinized during the early biotrophic growth of M. oryzae and then become acidified during the later necrotrophic growth. (B) During biotrophic growth, the PacC 559 and PacC 222 transcription factor isoforms localize to the nucleus, where PacC 559 acts as a transcriptional repressor to repress expression of genes associated with conidiation and necrotrophic growth, including PRG1 , HTF1 , and PIG1 while PacC 222 acts as a transcriptional activator to activate genes associated with biotrophic growth. (C) As host cells become acidified and lose viability, the PacC functional isoforms exit from the nucleus thereby de-repressing expression of genes related to necrotrophic growth and conidiation.

    Journal: bioRxiv

    Article Title: PacC-dependent adaptation and modulation of host cellular pH controls hemibiotrophic invasive growth and disease development by the rice blast fungus

    doi: 10.1101/2020.06.22.164590

    Figure Lengend Snippet: (A) Infected plant cells are alkalinized during the early biotrophic growth of M. oryzae and then become acidified during the later necrotrophic growth. (B) During biotrophic growth, the PacC 559 and PacC 222 transcription factor isoforms localize to the nucleus, where PacC 559 acts as a transcriptional repressor to repress expression of genes associated with conidiation and necrotrophic growth, including PRG1 , HTF1 , and PIG1 while PacC 222 acts as a transcriptional activator to activate genes associated with biotrophic growth. (C) As host cells become acidified and lose viability, the PacC functional isoforms exit from the nucleus thereby de-repressing expression of genes related to necrotrophic growth and conidiation.

    Article Snippet: GST-fused PacC 559 , PacC 222 and PacC 80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E. coli BL21DE3 and purified, as described in supplementary data.

    Techniques: Infection, Expressing, Functional Assay